Advances in Clinical and Experimental Medicine
2011, vol. 20, nr 4, July-August, p. 423–429
Publication type: original article
Language: English
Association of Interleukin 1β Gene and Interleukin 1 Receptor Antagonist Gene Polymorphisms and Gastric Cancer Risk
Związek między polimorfizmami genu interleukiny 1β i genu antagonisty receptora interleukiny 1 i zagrożenie rozwojem raka żołądka
1 Department of Medical Genetics, University of Medicine and Pharmacy, Craiova, Romania
2 Research Center in Gastroenterology and Hepatology, Craiova, Romania
3 Department of Molecular and Cellular Biology, University of Medicine and Pharmacy, Craiova, Romania
4 Department of Medical Semiology, University of Medicine and Pharmacy, Craiova, Romania
5 Department of General Surgery, Emergency Hospital of Craiova, Romania
Abstract
Background. Gastric cancer is the second leading cause of cancer death in both sexes worldwide and the prognosis of this malignancy remains very poor. Objectives,
Material and Methods. This study assesses the association between interleukin 1β gene (IL-1B) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms and gastric cancer susceptibility in the Romanian population. The authors investigated IL-1B –31T > C, IL-1B +3954C > T and IL1-RN +2018T > C polymorphisms in 103 subjects diagnosed with gastric cancer and 244 healthy controls. These polymorphisms were genotyped by allelic discrimination TaqMan PCR.
Results. In all gastric cancer cases, a significant association with an increased risk of gastric cancer was only observed for the IL1-RN +2018T > C polymorphism (OR 2.52, 95% CI: 1.06–6.01). A stratified analysis showed that IL1-RN +2018T > C was associated with an increased risk of gastric non-cardia adenocarcinoma (OR 2.77, 95% CI: 1.10–6.99) and intestinal type (OR 3.08, 95% CI: 1.17–8.11). The authors found no significant differences between gastric cancer cases and controls for IL1-B –31CC (OR 0.77, 95% CI: 0.32–1.84) and IL1-B +3954TT (OR 0.77, 95% CI:0.32–1.85) genotypes polymorphisms.
Conclusion. The results presented do not support the hypothesis that the IL-1B –31T > C and IL-1B +3954C > > T polymorphisms determine differences in gastric cancer risk among different individuals, but the IL1-RN +2018T > C polymorphism could contribute to gastric cancer risk in the Romanian population.
Streszczenie
Wprowadzenie. Rak żołądka jest drugą pod względem częstości przyczyną zgonów z powodu nowotworów u obu płci na całym świecie, a rokowanie nadal jest bardzo złe. Cel pracy, materiał i metody. Ocena związku między polimorfizmami genu interleukiny 1β (IL-1B) i genu antagonisty receptora interleukiny 1 (IL-1RN) a podatnością na raka w populacji Rumunii. Autorzy badali polimorfizmy IL-1B-31T > C, IL-1B +3954 C > T i IL1-RN +2018 T > C u 103 pacjentów z rozpoznanym rakiem żołądka i 244 zdrowych. Polimorfizmy genotypowano przeprowadzone metodą dyskryminacji alleli z użyciem sond TaqMan PCR.
Wyniki. We wszystkich przypadkach raka żołądka istotny związek ze zwiększonym ryzykiem tego raka obserwowano jedynie dla polimorfizmu IL1-RN +2018 T > C (OR 2,52, 95% CI: 1.06–6.01). Analiza warstwowa wykazała, że IL1-RN +2018 T > C wiązał się ze zwiększonym ryzykiem gruczolakoraka żołądka (OR 2,77, 95% CI: 1.10–6 0.99) i jelit (OR 3,08, 95% CI: 1.17–8 0.11). Autorzy nie stwierdzili istotnych różnic między przypadkami raka żołądka a grupą kontrolną dla polimorfizmów genotypów IL1-B-31CC (OR 0,77, 95% CI: 0.32-1 0.84) i IL1-B +3954 TT (OR 0,77, 95% CI: 0.32–1 0.85).
Wnioski. Przedstawione wyniki nie potwierdzają hipotezy, że polimorfizmy IL-1B-31T > C i IL-1B +3954 C > T określają różnice w ryzyku rozwoju raka żołądka wśród różnych osób, ale polimorfizm IL1-RN +2018 T > C może wpływać na zwiększone ryzyko raka żołądka w populacji Rumunii.
Key words
gastric cancer, interleukin, gene polymorphism, genotype, risk
Słowa kluczowe
rak żołądka, interleukina, polimorfizm genu, genotyp, ryzyko
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