Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2011, vol. 20, nr 1, January-February, p. 23–29

Publication type: original article

Language: English

Evaluation of the Beneficial Effects of Zataria Multiflora Boiss in Halothane-Induced Hepatotoxicity in Rats

Ocena korzystnego działania Zataria multiflora na hepatotoksyczność wywołaną halotanem u szczurów

Ehsanollah Sakhaee1,, Ladan Emadi2,, Omid Azari1,, Farideh Sistani Khanaman3,

1 Department of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran

2 Department of Basic Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran

3 School of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran

Abstract

Background. Halothane is one of the most important anesthetics. In some studies, hepatic injury was occasionally induced in animal models as a result of the interaction of halothane exposure. Zataria multiflora is a valuable medicinal plant in the Labiatae family that is distributed only in Iran, Pakistan and Afghanistan. Objective. In this study, we attempted to evaluate the beneficial effects of Zataria multiflora Boiss in halothaneinduced hepatotoxicity in rats.
Material and Methods. In the present study, thirty-two Wistar albino rats, weighing 180–200 g (3.5–4 months old), were divided into four different groups for 7 days as follows: control animals (Group C), which did not receive any treatment, the second group (Group Z) which received Zataria multiflora extract (800 p.p.m. in drinking water) during the experiment period (7 days), the third group (Group H) which was exposed to an anesthetic gas mixture (100% oxygen and 2% halothane, v/v) in a vaporizer using the semi-closed method for 2 hours on the 6th day, and the fourth group (Group T), Zataria multiflora-treated animals which received 5 days of pretreatment with Zataria multiflora and were exposed to the anesthetic gas mixture for 2 hours on the 6th day, and then Zataria multiflora was continued through the 7th day.
Results. Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and total bilirubin (T-BIL) were significantly increased (p < 0.05) by halothane anesthesia (Group H) as compared with group C. All of the aforementioned parameters except AST were significantly decreased (p < 0.05) in group T in comparison with group H.
Conclusion. Based upon our results, Zataria multiflora extract may have a prophylactic effect in the prevention of halothane hepatotoxicity complications.

Streszczenie

Wprowadzenie. Halotan jest jednym z najważniejszych środków znieczulających. W niektórych badaniach uszkodzenie wątroby wywołano przypadkowo na modelach zwierzęcych w wyniku oddziaływania halotanu. Zataria multiflora jest cenną rośliną leczniczą z rodziny Labiatae, która występuje tylko w Iranie, Pakistanie i Afganistanie.
Cel pracy. Ocena korzystnego działania rośliny Zataria multiflora na uszkodzenie wątroby u szczurów wywołane przez halotan.
Materiał i metody. W przeprowadzonym badaniu, trwającym 7 dni, 32 albinotyczne szczury szczepu Wistar o masie 180–200 g (3,5–4-miesięczne) zostały podzielone na 4 różne grupy na 7 dni w następujący sposób: zwierzęta z grupy kontrolnej (grupa C), które nie otrzymywały żadnego leczenia, druga grupa (grupa Z), która otrzymała ekstrakt Zataria multiflora (800 ppm w wodzie pitnej) podczas eksperymentu (7 dni), trzecia grupa (grupa H), która została poddana narkozie mieszaniną gazów znieczulających (100% tlenu i 2% halotanu, v/v) w parowniku metodą częściowo zamkniętą na 2 godz. przez 6 dni, a w czwartej grupie (grupa T) były zwierzęta, którym podawano Zataria multiflora; wstępnie otrzymywały Zataria multiflora przez 5 dni i 6. dnia poddano je działaniu mieszaniny gazów znieczulających przez 2 godz. , a 7. dnia kontynuowano podawanie im Zataria multiflora.
Wyniki. Stężenie aminotransferazy asparaginianu (AST), aminotransferazy alaninowej (AlAT), dehydrogenazy mleczanowej (LDH), fosfatazy alkalicznej (ALP) i stężenie bilirubiny całkowitej (T-BIL) były znacznie większe (p < 0,05) pod wpływem znieczulenia halotanem (grupa H) w porównaniu z grupą C. Wszystkie wyżej wymienione wskaźniki, z wyjątkiem AST, były istotnie mniejsze (p < 0,05) w grupie T w porównaniu z grupą H.
Wnioski. Na podstawie wyników badań autorzy uważają, że ekstrakt Zataria multiflora może wywierać korzystne działanie w zapobieganiu powikłaniom uszkodzeń wątroby wywołanych halotanem.

Key words

rat, halothane, Zataria multiflora, liver

Słowa kluczowe

szczur, halotan, Zataria multiflora, wątroba

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