Advances in Clinical and Experimental Medicine

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Advances in Clinical and Experimental Medicine

2010, vol. 19, nr 4, July-August, p. 437–441

Publication type: original article

Language: English

The Diagnostic Importance of Detecting Chlamydia Trachomatis Antigen and Anti-IgG Anti-cHSP60 Antibodies in Infertile Women

Znaczenie diagnostyczne wykrywania antygenu Chlamydia trachomatis i przeciwciał IgG anty cHSP60 u niepłodnych kobiet

Magdalena Frej-Mądrzak1,, Agnieszka Jama-Kmiecik1,, Irena Choroszy-Król1,

1 Department of Basic Sciences, Faculty of Health Sciences, Wroclaw Medical University, Poland

Abstract

Background. Clinical forms of chlamydia infection in women are inflammation of the urethra, urethral syndrome, cervicitis, inflammation of the rectum, and conjunctivitis. Persistence of Chlamydia trachomatis infection leads to an increased production of the 60-kDa chlamydial heat-shock protein (cHSP60).
Objectives. The aim of this study was to assess the diagnostic value and usefulness of the cHSP60-IgG-ELISA test and to determine the prevalence of IgG anti-cHSP60 antibodies in infertile women according to age groups.
Material and Methods. The study group consisted of 108 women with primary (n = 91; 84.3%) and secondary (n = 17; 15.7%) infertility. The control group (n = 37) consisted of women without any complaints of the urogenital tract. C. trachomatis antigen was determined in cervical smears by the direct immunofluorescence Pathfinder® Chlamydia trachomatis Direct Specimen test from Bio-Rad. IgG anti-cHSP60 antibodies were determined in the serum using the cHSP60-IgG-ELISA test from medac.
Results. The 108 cervical swabs of the women being treated due to infertility were labeled by direct immunofluorescence (DIF); a positive test result was noted in 41 (37.9%) patients. Serum from the 108 was assessed by ELISA and IgG anti-cHSP60 antibodies were found in 17 (15.7%). In the control group, positive results of the C. trachomatis test were found in 18.9% of the women by DIF and IgG anti-cHSP60 antibodies were found in the serum of 5 (15.6%).
Conclusion. Serological tests may only supplement bacteriological tests and have some value in epidemiological studies. However, they should not be used as an alternative to bacteriological examination.

Streszczenie

Wprowadzenie. Kliniczne postacie zakażeń Chlamydia trachomatis u kobiet to zapalenie cewki moczowej i zespół cewkowy, zapalenie szyjki macicy, odbytu oraz zapalenie spojówek. Przetrwałe zakażenie chlamydią skutkuje wzrostem wydzielania cHSP60 (chlamydialnego białka szoku termicznego o masie 60 kDa).
Cel pracy. Ocena wartości i przydatności diagnostycznej testu cHSP60-IgG-ELISA oraz określenie występowania przeciwciał IgG anty cHSP60 u niepłodnych kobiet z uwzględnieniem grup wiekowych.
Materiał i metody. Grupę badaną stanowiło 108 kobiet, u których zdiagnozowano niepłodność pierwotną (n = 91; 84,3%) lub niepłodność wtórną (n = 17; 15,7%). Grupę kontrolną stanowiły kobiety (n = 37) bez dolegliwości ze strony układu moczowo-płciowego, które zgłaszały się do poradni ginekologicznej w celu wykonania rutynowych badań profilaktycznych. Do badania antygenów C. trachomatis w wymazach z cewki moczowej i szyjki macicy metodą immunofluorescencji bezpośredniej (DIF – direct immunofluorescence) zastosowano test Pathfinder® Chlamydia trachomatis Direct Specimen, firmy Biorad. Do wykrywania przeciwciał w surowicy krwi badanych zastosowano test cHSP60-IgG-ELISA firmy medac.
Wyniki. Antygen Chlamydia trachomatis wykrywano metodą immunofluorescencji bezpośredniej (DIF) w 108 wymazach z kanału szyjki macicy; wyniki dodatnie uzyskano u 41 (37,9%) kobiet. 108 surowic badano z użyciem techniki ELISA i przeciwciała IgG anty-cHSP60 wykryto u 17 (15,7%). W grupie kontrolnej pozytywne wyniki badań w kierunku C. trachomatis uzyskano w 18,9% kobiet techniką DIF, a przeciwciała IgG anty-cHSP60 wykryto w surowicy 5 (15,6%) pacjentek.
Wnioski. Testy serologiczne mogą stanowić uzupełnienie testów bakteriologicznych, mają wartość epidemiologiczną. Nie powinny być jednak alternatywą standardowych badań bakteriologicznych.

Key words

Chlamydia trachomatis, IgG anti cHSP60

Słowa kluczowe

Chlamydia trachomatis, IgG anty cHSP60

References (21)

  1. Zdrodowska-Stefanow B, Ostaszewska I : Chlamydia trachomatis – zakażenia u ludzi. Volumed, Wrocław 2000.
  2. Kinnunen A: Chlamydial heat shock protein 60 and cell-mediated immunity in tubal factor infertility. Oulu, Finland 2002.
  3. Ness RB, Shen C, Bass D, Jackson C, Moysich K, Edwards R, Brunham RC: Chlamydia trachomatis serology in women with and without ovarian cancer. Infect Dis Obstet Gynecol 2008, 20, 1–5.
  4. Wallin KL, Wiklund F, Luostarinen T, Angström T, Anttila T, Bergman F, Hallmans G, Ikäheimo I, Koskela P, Lehtinen M, Stendahl U, Paavonen J, Dillner J: A population-based prospective study of Chlamydia trachomatis infection and cervical carcinoma. Int J Cancer 2002, 101, 371–374.
  5. Witkin SS, Linhares IM: Chlamydia trachomatis in subfertile women undergoing uterine instrumentation: an alternative to direct microbial testing or prophylactic antibiotic treatment. Hum Reprod 2002, 17, 1938–1941.
  6. Idahl A, Boman J, Kumlin U, Olofsson JI: Demonstration of Chlamydia trachomatis IgG antibodies in the male partner of the infertile couple is correlated with a reduced likelihood of achieving pregnancy. Hum Reprod 2004, 19, 1121–1126.
  7. Wilkowska-Trojmiel M, Zdrodowska-Stefanow B, Ostaszewska-Puchalska I, Redźko S, Przepieść J, Zdrodowski M: The influence of Chlamydia trachomatis infection on the spontaneus abortions. Adv Med Sci 2009, 54, 86-90.
  8. Witkin SS: Immunity to heat shock proteins and pregnancy outcome. Infect Dis Obstet Gynecol 1999, 7, 35–38.
  9. Witkin SS, Askienazy-Elbhar M, Henry-Suchet J, Belaisch-Allart J, Tort-Grumbach J, Sarjdine K: Circulating antibodies to a conserved epitope of the Chlamydia trachomatis 60 kDa heat shock protein (hsp60) in infertile couples and its relationship to antibodies to C. trachomatis surface antigens and the Escherichia coli and human HSP60. Hum Reprod 1998, 13, 1175–1179.
  10. Di Felice V, David S, Cappello F, Farina F, Zummo G: Is chlamydial heat shock protein 60 a risk factor for oncogenesis? Cell Mol Life Sci 2005, 62, 4–9.
  11. Eggert-Kruse W, Neuer A, Clussmann C, Boit R, Geissler W, Rohr G, Strowitzki T: Seminal antibodies to human 60kd heat shock protein (HSP 60) in male partners of subfertile couples. Hum Reprod 2002, 17, 726–735.
  12. Kozakiewicz B: Nowotwory złośliwe narządu rodnego. Nowa Med Onkol VI 2003, 3 http://www.czytelniamedyczna. pl/nm_on29.php.
  13. Neuer A, Spandorfer SD, Giraldo P, Dieterle S, Rosenwaks Z, Witkin SS: The role of heat shock proteins in reproduction. Hum Reprod Update 2000, 6, 149–159.
  14. Pospisil L, Stroblova H, Canderle J, Unzeitig V: The chlamydial heat shock protein (cHSP60) in females participating in fertilization programs (a serological study). Acta Dermatoven APA 2004, 13, 75–78.
  15. Paavonen J, Karunakaran KP, Noguchi Y, Anttila T, Bloigu A, Dillner J, Hallmans G, Hakulinen T, Jellum E, Koskela P, Lehtinen M, Thoresen S, Lam H, Shen C, Brunham RC: Serum antibody response to the heat shock protein 60 of Chlamydia trachomatis in women with developing cervical cancer. Am J Obstet Gynecol 2003, 189, 1287–1292.
  16. Clad A, Petersen EE, Böttcher M: Extended Chlamydia trachomatis serology: cHSP60 IgG and its association with tubal occlusion. Fifth Meeting of the European Society for Chlamydia Research, Budapest, Hungary, 2004, September 1–4
  17. Karinen L, Pouta A, Hartikainen AL, Bloigu A, Paldanius M, Leinonen M, Saikku P, Järvelin MR: Antibodies to Chlamydia trachomatis heat shock proteins Hsp60 and Hsp10 and subfertility in general population at age 31. Am J Reprod Immunol 2004, 52, 291–297.
  18. Dutta R, Jha R, Salhan S, Mittal A: Chlamydia trachomatis-specific heat shock proteins 60 antibodies can serve as prognostic marker in secondary infertile women. Infection 2008, 36, 374–378.
  19. Dutta R, Jha R, Gupta S, Gupta R, Salhan S, Mittal A: Seroprevalence of antibodies to conserved region of Chlamydia heat shock proteins 60 and 10 and their possible association with sex hormones in Chlamydia trachomatis infected women in India. Br J Biomem Sci 2007, 64, 78–83.
  20. Crha I, Pospísil L, Stroblová H, Ventruba P, Záková J, Huser M: Antibodies against the chlamydial heat shock protein in women with periadnexal adhesions. Ceska Gynekol 2006, 71, 127–131.
  21. Mascellino MT, Ciardi MR, Oliva A, Cecinato F, Hassemer MP, Borgese L: Chlamydia trachomatis detection in a population of asymptomatic and symptomatic women: correlation with the presence of serological markers for this infection. New Microbiol 2008, 31, 249–256.
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