Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2009, vol. 18, nr 3, May-June, p. 243–248

Publication type: original article

Language: English

A Population of Zinc Transporter 3−Like Immunoreactive Neurons is Present in the Ganglia of Human Descending Colon

Populacja neuronów immunoreaktywnych wobec trzeciego transportera cynku w śródściennych zwojach nerwowych okrężnicy zstępującej człowieka

Sławomir Gonkowski1,, Barbara Kamińska2,, Piotr Landowski2,, Cezary Skobowiat1,, Piotr Burliński1,, Mariusz Majewski1,3,, Jarosław Całka1,4,

1 Division of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland

2 Clinic of Pediatrics, Gastroenterology, and Oncology, Medical University, Gdańsk

3 Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland

4 Józef Rusiecki Olsztyn University College, Olsztyn, Poland

Abstract

Background. Zinc transporter 3 (ZnT3), a member of the SLC30 family of divalent cation transporters, has recently been reported to be present in the terminals of so−called “zinc−enriched neurons” (ZEN) located in both the central and sympathetic peripheral nervous system.
Objectives. The present study was aimed at disclosing whether ZnT3 may also be present in the enteric nervous system (ENS) of human descending colon.
Material and Methods. Tissues were collected from patients undergoing resection of the descending colon due to intestinal obstruction (n = 5) and subjected to double−label immunofluorescence using antisera directed towards protein gene product 9.5 (PGP 9.5, used here as a pan−neuronal marker) in combination with an antibody against ZnT3.
Results. ZnT3−like immunoreactive (ZnT3−LI) neurons were observed in each of the three studied plexuses. ZnT3−positive neurons constituted 31.75 ± 2.01% of all perikarya observed in the myenteric, 26.77 ± 1.09% in the outer submucous, and 24.36 ± 1.59% in the inner submucous plexus.
Conclusion. These data demonstrate for the first time ZnT3−like immunoreactivity in the ENS and suggest that ZnT3 and, consequently, ZEN terminals may participate in the neural control of human colonic activities.

Streszczenie

Wprowadzenie. Trzeci transporter cynku (ZnT3) jest przedstawicielem kationowych transporterów SLC 39, które biorą udział w przenoszeniu cynku z przestrzeni pozakomórkowej do cytoplazmy. ZnT3 był obserwowany w tzw. neuronach ZEN w centralnym i obwodowym układzie nerwowym.
Cel pracy. Stwierdzenie, czy ZnT3 jest obecny w neuronach jelitowego układu nerwowego okrężnicy zstępującej człowieka.
Materiał i metody. Materiał do badań uzyskano od pacjentów (n = 5) poddanych resekcji okrężnicy zstępującej z powodu niedrożności jelit. Wycinki okrężnicy wybarwiono z użyciem techniki immunofluorescencji podwójnej, stosując surowice skierowane przeciwko PGP 9.5 (użytemu tu jako marker panneuronalny) i ZnT3.
Wyniki. Neurony ZnT3−immunoreaktywne były obserwowane w trzech zwojach śródściennych jelitowego układu nerwowego okrężnicy. W zwoju mięśniówkowym (MP) stanowiły one 31,75 ± 2,01% wszystkich komórek nerwowych, w zwoju podśluzówkowym zewnętrznym (OSP) – 26,77 ± 1,09%, a w zwoju podśluzówkowym wewnętrznym (ISP) – 24,36 ± 1,59%.
Wnioski. Wniniejszych badaniach po raz pierwszy wykazano obecność ZnT3−immunoreaktywnych neuronów jelitowym układzie nerwowym człowieka, co sugeruje udział trzeciego transportera cynku i neuronów ZEN w nerwowej regulacji czynności jelit.

Key words

enteric nervous system (ENS), zinc transporter 3 (ZnT3), descending colon, immunohistochemistry

Słowa kluczowe

jelitowy układ nerwowy (ENS), trzeci transporter cynku (ZnT3), okrężnica zstępująca, immunohistochemia

References (30)

  1. Vallee BL, Falchuk KH: The biochemical basis of zinc physiology. Physiol Rev 1993, 73, 79–118.
  2. Dineley KE, Votyakova TV, Reynolds IJ: Zinc inhibition of cellular energy production: implications for mitochondria and neurodegenaration. J Neurochem 2003, 85, 563–570.
  3. Frederickson CJ, Suh SW, Silva D, Thompson RD: Importance of zinc in the central nervous system: the zinccontaining neuron. J Nutr 2000, 13, 1471S–1483S.
  4. Barañano DE, Ferris CD, Snyder SH: Atypical neural messengers. Trends Neurosci 2001, 24, 99–106.
  5. Jo SM, Danscher G, Schroeder HD, Won MH, Cole TB: Zinc−enriched (ZEN) terminals in mouse olfactory bulb. Brain Res 2000, 865, 227–236.
  6. Frederickson CJ, Rampy BA, Reamy−Rampy S, Howell GA: Distribution of histochemically reactive zinc in the forebrain of the rat. J Chem Neuroanat 1992, 5, 521–530.
  7. Perez−Clauselly J, Danscher G: Intravesicular localization of zinc in rat telencephalic boutons. A histochemical study. Brain Res 1985, 337, 91–98.
  8. Mancini M, Ricci A, Amenta F: Age−related changes in sulfide−silver staining in the rat neostriatum: a quantitative histochemical study. Neurobiol Aging 1992, 13, 501–504.
  9. Kozma M, Szerdahelyi P, Kasa P: Histochemical detection of zinc and copper in various neurons of the central nervous system. Acta Histochem 1981, 69, 12–17.
  10. Jo SM, Danscher G, Schrøder HD, Won MH, Cole TB: Zinc-enriched (ZEN) terminals in mouse spinal cord: immunohistochemistry and autometallography. Brain Res 2000, 870, 163–169.
  11. Wang ZY, Li JY, Danscher G, Dahlström A: Localization of zinc−enriched neurons in the mouse peripheral sympathetic system. Brain Res 2002, 928, 165–174.
  12. Wang ZY, Danscher G, Dahlstrom A, Li JY: Zinc transporter 3 and zinc ions in the rodent superior cervical ganglion neurons. Neuroscience 2003, 120, 605–616.
  13. Eide DJ: Zinc transporters and the cellular trafficking of zinc. Bioch Bioph Acta 2006, 1763, 711–722.
  14. Eide DJ: Molecular biology of iron and zinc uptake in eukaryotes. Curr Opin Cell Biol 1997, 9, 573–577.
  15. Eide DJ: The SLC39 family of metal ion transporters. Pflugers Arch 2004, 447, 796–800.
  16. Palmiter R, Huang L: Efflux and compartmentalization of zinc by members of the SLC30 family of solute carriers. Pflugers Arch 2004, 447, 744–751.
  17. Kambe T, Yamaguchi−IwaiY, Sasaki R, Nagao M: Overview of mammalian zinc transporters. Cell Mol Life Sci 2004, 61, 49–68.
  18. Wenzel HJ, Cole TB, Born DE, Schwartzkroin PA, Palmiter RD: Ultrastructural localization of zinc transporter−3 (ZnT3) to synaptic vesicle membranes within mossy fiber boutons in the hippocampus of mouse and monkey. Proc Natl Acad Sci USA 1997, 94, 12676–12681.
  19. Danscher G, Wang Z, Kim YK, Kim SJ, Sun Y, Jo SM: Immunocytochemical localization of zinc transporter 3 in the ependyma of the mouse spinal cord. Neurosci Lett 2003, 342, 81–84.
  20. Palmiter RD, Cole TB, Quaife CJ, Lindley SD: ZnT−3, a putative transporter of zinc into synaptic vesicles. Proc Natl Acad Sci USA 1996, 93, 14934–14939.
  21. Danscher G, Jo SM, Varea E, Wang Z, Cole TB, Schrøder HD: Inhibitory Zinc-enriched terminals in mouse spinal cord. Neuroscience 2001, 105, 941–947.
  22. Wang Z, Danscher G, Kim YK, Dahlstrom A, Jo SM: Inhibitory zinc−enriched terminals in the mouse cerebellum: double−immunohistochemistry for zinc transporter 3 and glutamate decarboxylase. Neurosci Lett 2002, 321, 37–40.
  23. Molnar P, Nadler JV: Lack of effects of mossy fiber−released zinc on granule cell GABA (A) receptors in the pilocarine model of epilepsy. J Neurophysiol 2001, 85, 1932–1940.
  24. Koh JY, Suh SW, Gwag BJ, He YY, Hsu CY, Choi DW: The role of zinc in selective neuronal death following transient global cerebral ischemia. Science 1996, 272, 1013–1016.
  25. Suh SW, Chen JW, Motamedi M, Bell B, Listiak K, Pons NF, Danscher G, Frederickson CJ: Evidence that synaptically−related zinc contributes to neuronal injury after traumatic brain injury. Brain Res 2000, 852, 268–273.
  26. Takeda A: Movement of zinc and its functional significance in the brain. Brain Res Rev 2000, 34, 137–148.
  27. Giaroni C, Zanetti E, Chiaravalli AM, Albarello L, Dominioni L, Capella C, Lecchini S, Frigo G: Evidence for a glutamatergic modulation of the cholinergic function in the human enteric nervous system via NMDA receptors. Eur J Pharm 2003, 476, 63–69.
  28. Liu MT, Rothstein JD, Gershon MD, Kirchgessner A: Glutamatergic enteric neurons. J Neurosci 1997, 17, 4764–4784.
  29. Kirchgessner A: Glutamate in the enteric nervous system. Curr Opin Pharm 2001, 1, 591–596.
  30. Wang Z, Li JY, Dahlström A, Danscher G: Zinc−enriched GABAergic terminals in mouse spinal cord. Brain Res 2001, 921, 165–172.
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