Advances in Clinical and Experimental Medicine

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Advances in Clinical and Experimental Medicine

2009, vol. 18, nr 1, January-February, p. 33–39

Publication type: original article

Language: English

Differential Expression of CD34, S100, and c−Kit in Interstitial Cells of Cajal in Infantile Hypertrophic Pyloric Stenosis – Immunochemical Study

Różnicowa ekspresja CD34, S100 i c−Kit w komórkach śródmiąższowych Cajala we wrodzonym przerostowym zwężeniu odźwiernika – badanie immunochemiczne

Hulya Ozturk1,, Hayrettin Ozturk2,, Fahri Yilmaz3,, Hanifi Okur4,, Selcukotc Selcukotcu5,, Ali Ihsan Dokucu6,

1 Duzce University, Medical School, Department of Pediatric Surgery, Duzce, Turkey

2 Abanty Izzet Baysal University, Medical School, Departments of Pediatric Surgery, Bolu, Turkey

3 Abanty Izzet Baysal University, Medical School, Departments of Pediatric Surgery, Pathology2, Bolu, Turkey

4 Dicle University, Medical School, Department of Pediatric Surgery, Diyarbakir, Turkey

6 Sisli Etfal Training and Research Hospital, Department of Pediatric Surgery3, Istanbul, Turkey

Abstract

Background. The pathogenesis of infantile hypertrophic pyloric stenosis (IHPS) is poorly understood although many hypotheses have been proposed.
Objectives. Assessment whether the differential expression of c−Kit, CD34, and S100 may be involved in the development of IHPS.
Material and Methods. Specimens from 14 infants with IHPS and seven control subjects were immunohistochemically stained for c−Kit, CD34, and S100. The numbers of CD34+, S100+, and c−Kit+ cells in five random fields per specimen were compared via light microscopy (×200).
Results. In normal pyloric tissue, specific and intense c−Kit immunoreactivity was observed in the muscle layers and moderate staining was observed around the myenteric plexus. In IHPS patients, c−Kit+ cells were either absent or markedly reduced around the myenteric plexus. In control and IHPS patients, CD34+ cells were not observed around the myenteric plexus. In the vascular endothelium, moderate CD34 staining was observed in specimens from control subjects, whereas intense staining was observed for IHPS patients. In normal pyloric tissue, moderate S100 immunoreactivity was observed in the muscle layers and intense staining was observed in the myenteric plexus. In IHPS patients, few S100+ cells were observed in the pyloric muscle layers and S100 immunoreactivity decreased markedly around the myenteric plexus.
Conclusion. These results suggest that the numbers of c−Kit+ and S100+ cells are markedly decreased in the pyloric muscle layers and around the myenteric plexus in IHPS patients. Thus a lack of c−Kit and S100, but not CD34, expression may be a critical factor in the pathogenesis of IHPS and may serve as a useful prognostic tool in the treatment of this disease.

Streszczenie

Wprowadzenie. Patogeneza wrodzonego przerostowego zwężenia odźwiernika (w.p.z.o.) nie została dostatecznie poznana, chociaż zaproponowano wiele hipotez.
Cel pracy. Ocena znaczenia ekspresji różnicowej c−Kit, CD34 i S100 w rozwoju w.p.z.o.
Materiał i metody. Przeprowadzono badania immunohistochemicznie ekspresji c−Kit, CD34 oraz S100 w wycinkach tkankowych pobranych od 14 niemowląt cierpiących na w.p.z.o. i od 7 osób z grupy kontrolnej. W obu grupach porównano liczbę komórek CD34+, S100+ i komórek c−Kit+ z 5 losowo wybranych obszarów z każdego wycinka za pomocą mikroskopu optycznego (×200).
Wyniki. W prawidłowej tkance odźwiernika stwierdzono swoistą i silną immunoreaktywność c−Kit w warstwach mięśniowych. Nie stwierdzono komórek CD34+ w okolicy splotów nerwowych błony mięśniowej, a w okolicy splotów nerwowych błony mięśniowej barwienie było umiarkowane. U pacjentów z w.p.z.o. komórki c−Kit+ były albo nieobecne, albo było ich znacząco mniej w okolicy splotów nerwowych błony mięśniowej zarówno w grupie kontrolnej, jak i u pacjentów z w.p.z.o. W śródbłonku naczyń krwionośnych zaobserwowano umiarkowane barwienie CD34 w wycinkach pochodzących z grupy kontrolnej, a w wycinkach pochodzących od pacjentów z w.p.z.o. barwienie było intensywne. W prawidłowej tkance odźwiernika obserwowano umiarkowaną immunoreaktywność S100 w warstwach mięśniowych oraz intensywne barwienie w okolicy splotów nerwowych błony mięśniowej. U pacjentów z w.p.z.o. wykryto niewiele komórek S100+ w warstwie mięśniowej odźwiernika oraz zmniejszoną reaktywność S100 w okolicy splotów nerwowych błony mięśniowej.
Wnioski. Przedstawione wyniki sugerują, że liczba komórek c−Kit+ i S100+ jest znacząco mniejsza w warstwach mięśniowych odźwiernika oraz w okolicy splotów nerwowych błony mięśniowej pacjentów z w.p.z.o. Dlatego brak ekspresji c−Kit i S100, ale nie CD34 może być uznany za ważny czynnik patogenetyczny w.p.z.o. i może być pożytecznym narzędziem diagnostycznym w leczeniu tej choroby.

Key words

infantile hypertrophic pyloric stenosis (IHPS), CD34, S100, c−Kit, immunohistochemistry

Słowa kluczowe

wrodzone przerostowe zwężenie odźwiernika, CD34, S100, c−Kit, immunohistochemia

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