Advances in Clinical and Experimental Medicine
2008, vol. 17, nr 2, March-April, p. 213–216
Publication type: original article
Language: English
Calcineurin Inhibitors Alter the Osmotic Properties of Erythrocytes in Patients After Renal Transplantation
Inhibitory kalcyneuryny zmieniają własności osmotycznych erytrocytów u chorych po przeszczepie nerki
1 Institute of Physics, Wroclaw University of Technology, Poland
2 Department of Nephrology and Transplantation Medicine, Silesian Piasts University of Medicine in Wrocław, Poland
3 Faculty of Chemistry, University of Wroclaw, Poland
Abstract
Background. Renal transplant recipients treated with calcineurin inhibitors are known to demonstrate abnormalities in the physical properties of red blood cell membranes. Objects. The aim of the present study was to determine whether the osmotic properties of erythrocytes are altered in patients after renal transplantation treated with cyclosporin A (CsA) or tacrolimus (TAC).
Material and Methods. Venous blood samples were collected from 34 renal transplant recipients with good allograft function (serum creatinine concentration < 1.3 mg/dl). Among them, 13 were treated with tacrolimus and 21 with cyclosporin. Erythrocytes from 31 healthy donors were also examined.
Results. Osmotic fragility (osmolarity at 50% hemolysis) was determined spectrophotometrically in solutions of varying NaCl concentration. The rate of hemolysis was determined in hypotonic solution (62 mM NaCl) by the stopped−flow method. CsA and TAC cause the migration of the hemolysis curve towards lower osmolarities. Statistically significant decreases in relative osmotic fragility were observed both in patients receiving CsA patients compared with controls (0.409 ± 0.004 vs. 0.420 ± 0.003) %NaCl and in those receiving TAC (0.407 ± 0.004 vs. 0.420 ± 0.003) %NaCl. A statistically significant decrease in the rate of hemolysis during tacrolimus treatment (0.103 ± 0.027 vs. 0.127 ± 0.015) s–1 was found. There was negative correlation between cyclosporin through level and osmotic hemolysis rate, but no such correlation for tacrolimus vasseen.
Conclusion. These results suggest that both calcineurin inhibitors alter the osmotic properties of erythrocytes towards decreased susceptibility to hypotonic hemolysis.
Streszczenie
Wprowadzenie. Leczenie inhibitorami kalcyneuryny wpływa na fizyczne własności błon erytrocytów biorców przeszczepu nerki.
Cel pracy. Zbadanie, czy zmiany własności osmotycznych błony erytrocytów zachodzą po zastosowaniu różnych inhibitorów kalcyneuryny u biorców przeszczepu nerki leczonych cyklosporyną lub takrolimusem. Materiały i metody. Krew żylną pobrano od 34 biorców przeszczepu nerki z dobrą czynnościa przeszczepu (stężenie kreatyniny w surowicy poniżej 1,3 mg/dl). 13 chorych pobierało takrolimus, a 21 cyklosporynę. Do badań kontrolnych użyto erytrocyty od 31 zdrowych ochotników. Do badania szybkości hemolizy osmotycznej w roztworze hipotonicznym (62 mM NaCl) wykorzystano metodę pomiaru kinetyki szybkich reakcji (stopped flow). Podatność osmotyczną (osmolarność przy 50% hemolizie) wyznaczono spektrofotometrycznie w roztworach o różnych stężeniach NaCl.
Wyniki. Cyklosporyna i takrolimus wpływały na przesunięcie krzywej hemolizy w kierunku niższych osmolarności. Istotne zmniejszenie podatności osmotycznej obserwowano u chorych leczonych cyklosporyną w stosunku do osób zdrowych (0,409 ± 0,004 vs 0,420 ± 0,003) %NaCl i takrolimusem (0,407 ± 0,004 vs 0,420 ± 0,003) %NaCl. Obserwowano istotne zmniejszenie szybkości hemolizy podczas leczenia takrolimusem (0,103 ± 0,027 vs 0,127 ± 0,015)(s–1).
Wnioski. Oba inhibitory kalcyneuryny zmieniają własności osmotyczne erytrocytów, zmniejszając podatność na osmotyczną hemolizę.
Key words
erythrocytes, renal transplantation, osmotic fragility, hemolysis, calcineurin inhibitors
Słowa kluczowe
erytrocyty, inhibitory kalcyneuryny, hemoliza, przeszczepianie nerek, podatność osmotyczna
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