Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 5, May, p. 581–585

doi: 10.17219/acem/118843

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Evaluation of the association between angiotensin converting enzyme insertion/deletion polymorphism and the risk of endometrial cancer in and characteristics of Polish women

Grzegorz Raba1,A,B,C,D,E,F, Izabela Zawlik1,2,A,C,D,E,F, Marcin Braun3,C,E,F, Sylwia Paszek2,E,F, Natalia Potocka2,B,E,F, Marzena Skrzypa2,E,F, Bogdan Obrzut4,5,B,E, Marek Kluza1,B,E, Katarzyna Kluza1,B,E, Barbara Zych6,E,F, Magdalena Janowska7,B,E, Tomasz Kluz1,A,B,E

1 Institute of Medical Sciences, College of Medical Sciences, University of Rzeszów, Poland

2 Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, College of Medical Sciences, University of Rzeszów, Poland

3 Department of Pathology, Chair of Oncology, Medical University of Lodz, Poland

4 College of Medical Sciences, University of Rzeszów, Poland

5 Department of Gynecology and Obstetrics, Provincial Clinical Hospital No. 2 in Rzeszów, Poland

6 Institute of Health Sciences, College of Medical Sciences, University of Rzeszów, Poland

7 Gynecology and Obstetrics Clinic, Frederic Chopin Clinical Provincial Hospital No. 1, Rzeszów, Poland


Background. Endometrial cancer is the most common malignant neoplasm of the female reproductive organs. A dysfunctional endometrial renin-angiotensin system (RAS) might contribute to the growth and spread of endometrial cancer. The RAS-related gene polymorphisms, including the polymorphism of insertion/deletion (I/D) in the angiotensin-converting enzyme (ACE) gene, influence RAS activity.
Objectives. In the present study, we examined the association between the I/D polymorphism of the ACE gene and endometrial cancer risk in Polish women.
Material and Methods. Genotype analysis of the ACE I/D polymorphism was carried out using polymerase chain reaction (PCR) on 142 endometrial cancer type 1 patients and 68 cancer-free subjects. The results of the analyses were correlated with clinical data.
Results. The frequency of DD, DI and II ACE genotypes did not vary significantly between the experimental group and the control group (40 (28%), 61 (43%) and 41 (29%) vs 18 (26%), 31 (46%), and 19 (28%), respectively; p = 0.935). In addition, the incidence of the DD, DI and II polymorphisms in the ACE gene did not vary significantly between the experimental subgroups when stratified by cancer grade – G1, G2 and G3 endometrioid carcinoma – and the control group. Furthermore, the ACE polymorphism was not significantly associated with hypertension, diabetes or lymph node metastasis.
Conclusion. The ACE I/D gene polymorphism was not associated with endometrial cancer risk or the clinicopathological features in Polish women.

Key words

endometrial cancer, molecular biology, ACE gene polymorphism

References (23)

  1. Bokhman JV. Two pathogenetic types of endometrial carcinoma. Gynecol Oncol. 1983;15(1):10–17.
  2. Gorzelniak K, Janke J, Engeli S, Luft FC, Sharma AM. Upregulation of the AT(1) receptor human adipocytes by hydrocortisone. J Hypertens. 2002;20:S43–S43.
  3. Rigat B, Hubert C, Alhencgelas F, Cambien F, Corvol P, Soubrier F. An insertion deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest. 1990;86(4):1343–1346.
  4. Li CB, Ansari R, Yu ZM, Shah D. Definitive molecular evidence of renin-angiotensin system in human uterine decidual cells. Hypertension. 2000;36(2):159–164.
  5. Deshayes F, Nahmias C. Angiotensin receptors: A new role in cancer? Trends Endocrinol Metabol. 2005;16(7):293–299.
  6. Lever AF, Hole DJ, Gillis CR, et al. Do inhibitors of angiotensin-I-converting enzyme protect against risk of cancer? Lancet. 1998;352(9123):179–184.
  7. Koyama N, Nishida Y, Ishii T, Yoshida T, Furukawa Y, Narahara H. Telmi­sartan induces growth inhibition, DNA double-strand breaks and apoptosis in human endometrial cancer cells. PLoS One. 2014;9(3):e93050.
  8. Piastowska-Ciesielska AW, Pluciennik E, Wojcik-Krowiranda K, Bienkiewicz A, Bednarek A, Ochedalski T. Analysis of the expression of angiotensin II type 1 receptor and VEGF in endometrial adenocarcinoma with different clinicopathological characteristics. Tumor Biol. 2012;33(3):767–774.
  9. Teranishi J, Yamamoto R, Nagasawa Y, et al. ACE insertion/deletion polymorphism (rs1799752) modifies the renoprotective effect of renin-angiotensin system blockade in patients with IgA nephropathy. J Renin Angiotensin Aldosterone Syst. 2015;16(3):633–641.
  10. Singh A, Srivastava N, Amit S, Prasad SN, Misra MP, Ateeq B. Association of AGTR1 (A1166C) and ACE (I/D) polymorphisms with breast cancer risk in North Indian population. Transl Oncol. 2018;11(2):233–242.
  11. Medeiros R, Vasconcelos A, Costa S, et al. Linkage of angiotensin I-converting enzyme gene insertion/deletion polymorphism to the progression of human prostate cancer. J Pathol. 2004;202(3):330–335.
  12. Yuan F, Zhang L-S, Li H-Y, Liao M, Lv M, Zhang C. Influence of angiotensin I-converting enzyme gene polymorphism on hepatocellular carcinoma risk in China. DNA Cell Biol. 2013;32(5):268–273.
  13. Freitas-Silva M, Pereira D, Coelho C, Bicho M, Lopes C, Medeiros R. Angiotensin I-converting enzyme gene insertion/deletion polymorphism and endometrial human cancer in normotensive and hypertensive women. Cancer Genet Cytogenet. 2004;155(1):42–46.
  14. Lahiri DK, Nurnberger JI. A rapid nonenzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res. 1991;19(19):5444.
  15. Sanhueza JA, Zambrano T, Bahamondes-Avila C, Salazar LA. Association of anxiety-related polymorphisms with sports performance in Chilean long distance triathletes: A pilot study. J Sports Sci Med. 2016;15(4):554–561.
  16. Vairaktaris E, Yapijakis C, Tsigris C, et al. Association of angiotensin-converting enzyme gene insertion/deletion polymorphism with increased risk for oral cancer. Acta Oncol. 2007;46(8):1097–1102.
  17. Zhang K, Cheng D, Yi L, Shi H, Zhen G. Association between angiotensin I-converting enzyme gene polymorphism and susceptibility to cancer: A meta-analysis. Int J Clin Exp Pathol. 2014;7(9):6291–6300.
  18. Wang Z-Y, Li H-Y, Jiang Z-P, Zhou T-B. Relationship between angiotensin-converting enzyme insertion/deletion gene polymorphism and prostate cancer susceptibility. J Cancer Res Ther. 2018;14(Suppl):S375–S380.
  19. Li X-L, Zheng Z-J, Qu H-O. Lack of association of angiotensin-converting enzyme insertion/deletion polymorphism with breast cancer: An update meta-analysis based on 10405 subjects. J Renin Angiotensin Aldosterone Syst. 2015;16(4):1095–1100.
  20. Wang N, Yang D, Ji B, Li J. Angiotensin-converting enzyme insertion/deletion gene polymorphism and lung cancer risk: A meta-analysis. J Renin Angiotensin Aldosterone Syst. 2015;16(1):189–194.
  21. Alves Correa-Noronha SA, Ribeiro de Noronha SM, Alecrim C, et al. Association of angiotensin-converting enzyme I gene I/D polymorphism with endometrial but not with ovarian cancer. Gynecol Endocrinol. 2012;28(11):889–891.
  22. Xie Y, You C, Chen J. An updated meta-analysis on association between angiotensin I-converting enzyme gene insertion/deletion polymorphism and cancer risk. Tumor Biol. 2014;35(7):6567–6579.
  23. Li P, Cao L, Han X. Angiotensin-converting enzyme (ACE) I/D polymorphism is a risk factor of allergic rhinitis. Cell Moll Biol. 2017;63(8):48–50.