Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 2, March-April, p. 219–226

doi: 10.17219/acem/33250

Publication type: original article

Language: English

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The Protective Effect of Adalimumab on Renal Injury in a Model of Abdominal Aorta Cross-Clamping

Medine C. Cure1,A,C,D,F, Erkan Cure2,A,B,C,D, Yildiray Kalkan3,C,D,F, Levent Tumkaya3,B,E,F, Ibrahim Aydin4,A,B,E, Aynur Kirbas1,A,B,F, Hasan Efe1,A,E,F, Aysel Kurt5,B,C,E, Suleyman Yuce2,B,C,F

1 Department of Biochemistry, Recep Tayyip Erdogan University, Turkey

2 Department of Internal Medicine, Recep Tayyip Erdogan University, Turkey

3 Department of Histology and Embrylogy, Recep Tayyip Erdogan University, Turkey

4 Department of Surgery, Recep Tayyip Erdogan University, Turkey

5 Department of Thoracic Surgery, Recep Tayyip Erdogan University, Turkey


Background. Adalimumab (ADA) is a potent inhibitor of tumor necrosis factor (TNF-α). ADA treatment suppresses proinflammatory cytokines, leading to a decrease or inhibition of the inflammatory process.
Objectives. The aim of this study was to investigate the possible protective effects of ADA on oxidative stress and cellular damage on rat kidney tissue after ischemia/reperfusion (I/R).
Material and Methods. A total of 30 male Wistar albino rats were divided into three groups: control, I/R, and I/R plus ADA (I/R + ADA); each group comprised 10 animals. The control group underwent laparotomy without I/R injury. After undergoing laparotomy, I/R groups underwent two hours of infrarenal abdominal aortic cross ligation, which was followed by two hours of reperfusion. ADA (50 mg/kg) was administered as a single dose, intraperitoneally, to the I/R + ADA group, 5 days before I/R.
Results. The I/R group’s TNF-α (1150.9 ± 145.6 pg/mg protein), IL-1β (287.0 ± 32.4 pg/mg protein) and IL-6 (1085.6 ± 56.7 pg/mg protein) levels were significantly higher than those of the control (916.1 ± 88.7 pg/mg protein, p = 0.003; 187.5 ± 37.2 pg/mg protein, p < 0.001; 881.4 ± 57.1 pg/mg protein, p < 0.001, respectively) and I/R + ADA groups (864.2 ± 169.4 pg/mg protein, p = 0.003; 241.4 ± 33.4 pg/mg protein, p = 0.010; 987.7 ± 66.5 pg/mg protein, p = 0.004, respectively). To date, a few histopathological changes have been reported regarding renal I/R injury in rats due to ADA treatment whereas I/R caused severe histopathological injury to kidney tissue.
Conclusion. ADA treatment significantly attenuated the severity of kidney I/R injury, inhibiting I/R-induced oxidative stress and renal damage. Because of its anti-inflammatory and antioxidant effects, ADA pretreatment may have protective effects on experimental kidney injury.

Key words

ischemia, reperfusion injury, adalimumab, carbonic anhydrases, tumor necrosis factor alpha

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