Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 3, May-June, p. 409–417

doi: 10.17219/acem/43761

Publication type: original article

Language: English

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The Protective Effects of Epigallocatechin Gallate Against Distant Organ Damage After Severe Skin Burns – Experimental Study Using a Rat Model of Thermal Trauma

Mubin Hosnuter1,A,E,F, Cenk Melikoglu2,B,C, Cem Aslan1,B,C,D, Gulcan Saglam3,B,C, Recep Sutcu3,B,C

1 Department of Plastic, Reconstructive and Aesthetic Surgery, Katip Celebi University School of Medicine, Ataturk Training and Research Hospital, Izmir, Turkey

2 Department of Plastic, Reconstructive and Aesthetic Surgery, Sifa University School of Medicine, Izmir, Turkey

3 Department of Biochemistry, Katip Celebi University School of Medicine, Ataturk Training and Research Hospital, Izmir, Turkey

Abstract

Background. Epigallocatechin gallate (EGCG), a green tea polyphenol, has potent antioxidant properties.
Objectives. The purpose of the present study was to examine the possible preventative effects of EGCG against internal organ injury due to large-surface skin burns in a rat model.
Material and Methods. The study design involved three groups of rats: a sham group and two groups with 25–30% full-thickness burns: (a) the sham group without burns or treatment (n = 18); (b) the control burn group (burns + sterile saline, n = 18); and (c) the burn treatment group (burns + treatment with EGCG, n = 18). EGCG was administered intraperitoneally immediately after the thermal injury, and daily in 100 μmol/kg doses. Kidney and lung tissue samples were taken to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), and glutathione peroxidase (GPX) after the first, third and seventh post-burn days.
Results. In the EGCG-treated burn group, SOD and GPX activity were significantly higher than in the burn control group. Additionally, MDA and TNF-α levels were significantly lower in the EGCG-treated burn group.
Conclusion. Based on this study, it might be anticipated that EGCG treatment may be beneficial in burn injury cases.

Key words

antioxidant, EGCG, free radicals, green tea, polyphenol.

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