Advances in Clinical and Experimental Medicine
2015, vol. 24, nr 3, May-June, p. 409–417
Publication type: original article
The Protective Effects of Epigallocatechin Gallate Against Distant Organ Damage After Severe Skin Burns – Experimental Study Using a Rat Model of Thermal Trauma
1 Department of Plastic, Reconstructive and Aesthetic Surgery, Katip Celebi University School of Medicine, Ataturk Training and Research Hospital, Izmir, Turkey
2 Department of Plastic, Reconstructive and Aesthetic Surgery, Sifa University School of Medicine, Izmir, Turkey
3 Department of Biochemistry, Katip Celebi University School of Medicine, Ataturk Training and Research Hospital, Izmir, Turkey
Background. Epigallocatechin gallate (EGCG), a green tea polyphenol, has potent antioxidant properties.
Objectives. The purpose of the present study was to examine the possible preventative effects of EGCG against internal organ injury due to large-surface skin burns in a rat model.
Material and Methods. The study design involved three groups of rats: a sham group and two groups with 25–30% full-thickness burns: (a) the sham group without burns or treatment (n = 18); (b) the control burn group (burns + sterile saline, n = 18); and (c) the burn treatment group (burns + treatment with EGCG, n = 18). EGCG was administered intraperitoneally immediately after the thermal injury, and daily in 100 μmol/kg doses. Kidney and lung tissue samples were taken to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), and glutathione peroxidase (GPX) after the first, third and seventh post-burn days.
Results. In the EGCG-treated burn group, SOD and GPX activity were significantly higher than in the burn control group. Additionally, MDA and TNF-α levels were significantly lower in the EGCG-treated burn group.
Conclusion. Based on this study, it might be anticipated that EGCG treatment may be beneficial in burn injury cases.
antioxidant, EGCG, free radicals, green tea, polyphenol.
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