Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1 (5-Year IF – 2.0)
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2013, vol. 22, nr 6, November-December, p. 825–829

Publication type: original article

Language: English

The Effect of A2A Receptor Antagonist (SCH 442416) on the mRNA Expression of Kir 2.1 and Kir 4.1 Channels in Rat Retinal Müller Cells Under Hypoxic Conditions in Vitro

Wpływ antagonisty receptora A2A (SCH 442416) na ekspresję mRNA kanałów Kir 2.1 i Kir 4.1 w komórkach Müllera u szczurów w warunkach hipoksji in vitro

Xiaoli Kang1,A,B,C,D, Jun Yu1,C,D, Yan Wei1,B,F, Peiquan Zhao1,E,F

1 Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Abstract

Objectives. To investigate the situation of inwardly rectifying potassium channels Kir 2.1 and Kir 4.1 under hypo­ xic conditions, and whether the A2A receptor antagonist could modulate the mRNA expression of Kir 2.1 and Kir 4.1 channels in retinal Müller cells in vitro under hypoxic conditions.
Material and Methods. Müller cells were treated with 0.1 1, 10 and 100 µM of A2A receptor antagonist (SCH 442416) under hypoxic conditions for 24 h, and the expression of the Kir 2.1 and Kir 4.1 mRNA channels was examined using real­time polymerase chain reaction (qPCR).
Results. There were no significant changes in the mRNA expression of the Kir 2.1 or Kir 4.1 channels under hypoxic conditions compared with normal conditions after 24 h cultured in vitro. The mRNA expression of Kir 2.1 and Kir 4.1 channels treated with 0.1 µM SCH 442416 under hypoxic conditions were increased, but at higher concentrations of SCH 442416, the mRNA expression of the Kir 2.1 and Kir 4.1 channels decreased.
Conclusion. The A2A receptor antagonist (SCH 442416) could increase mRNA expression of the Kir 2.1and Kir 4.1 channels in Müller cells to protect the retinal neurons in vitro under hypoxic conditions

Key words

Müller cells, Kir 4.1 channels, Kir 2.1 channels, A2A receptor antagonist, hypoxia.

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