Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

Ahead of print

doi: 10.17219/acem/161161

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Wei G, Tan M, Wang C, Liang L. Decreased miR-127 promotes the occurrence of breast cancer via increasing the expression of SPP1 [published online as ahead of print on March 15, 2023]. Adv Clin Exp Med. 2023. doi:10.17219/acem/161161

Decreased miR-127 promotes the occurrence of breast cancer via increasing the expression of SPP1

Guijiang Wei1,2,3,B,D, Meiying Tan1,2,3,A,B, Chunfang Wang1,2,3,C,E, Lina Liang1,2,3,A,F

1 Center for Clinical Laboratory Diagnosis and Research, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

2 Baise Key Laboratory of Clinical Molecular Diagnosis, Research and Development for High Incidence Diseases, China

3 Key Laboratory of Clinical Molecular Diagnosis and Research for High Incidence Diseases in Western Guangxi Universities, Baise, China

Abstract

Background. The expression of miR-127 has been reported to be decreased in the breast tissue of patients with breast cancer (BRC). However, the mechanism of miR-127 involvement in the pathogenesis of BRC is still unclear and requires urgent clarification.
Objectives. To explore the role of miR-127 in the pathogenesis of BRC.
Material and Methods. In this study, we measured the expression of miR-127 in blood samples of 60 BRC patients and 60 controls, investigated the influence of miR-127 on the viability and apoptosis of MCF-7 and MDA-231 cells, identified a miR-127 target gene, and determined the expression level of the target gene in the blood samples of BRC patients and controls.
Results. We found that miR-127 expression was significantly decreased in the plasma of BRC patients compared to controls. Additionally, the upregulation of miR-127 in MCF-7 and MDA-231 cells inhibited their proliferation and promoted their apoptosis. Conversely, the downregulation of miR-127 promoted cell proliferation and inhibited their apoptosis. The SPP1 was successively predicted and validated as a target gene of miR-127. Finally, the expression level of SPP1 was significantly increased in the plasma of BRC patients compared to controls.
Conclusion. Our study demonstrated that decreased miR-127 may promote BRC cell proliferation, inhibit apoptosis and promote the occurrence of BRC through increasing the SPP1 expression level.

Key words

pathogenesis, breast cancer, SPP1, miR-127

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